Bento Biotech 101 PCR for Lactose Intolerance Gene https://bento.bio/protocol/biotechnology-101/lactose-intolerance/

0. Preparation

PCR

• 5x FIREPol® Master Mix Ready To Load
• PCR Grade Water
• Primer Mix, Lactose Intolerance
• Ice
• Pipette 2 - 20µL
• Pipette Tips
• Microcentrifuge (Eppendorf) Tubes
• PCR tubes

Gel Electrophoresis

• Agarose Tablet (1)
• GelGreen® DNA Stain
• Gel Loading Dye
• 100 bp DNA Ladder
• Prepared 0.5x TBE buffer (prepared in Introduction to Gel Electrophoresis)
• Household table salt.