Video: https://vimeo.com/696442612

CRISPR_BioHacking_Waag2022.pdf

How to design plasmids? How do design CRISPR guide RNAs?

Reminder of “Central Dogma”

• Functional enzymes
• Catalyse
• Structural, Cell Walls
• etc.

Reading Frame

• 3 Base Pairs (= Codon) are translated to one Amino Acid
• Frame Shift Errors
• STOP Codon, does not translate to Amino Acid

CRISPR Edits

CRISPR makes a cut, different variants of CRISPR can make:

• Double-stranded Cut
• Single-stranded Cut