Protocol

https://bento.bio/protocol/biotechnology-101/dna-extraction-from-saliva/

Equipment & Reagents

Protocol

  1. Label 1.5m Eppendorf Tube with your name
  2. Prepare Saline (=Salt) solution Use ca. 5ml of water, mix with a pinch of salt
  3. Rinse your mouth
  4. Transfer to 1.5ml Eppendorf Tube. If you use a Paper Cup, bend it and fill the tube
  5. Centrifuge at 4KG for 90sec
  6. Recover the Pellet by decanting the Supernatant The Pellet is the white concentration of cells on the bottom of the tube. The liquid above is the Supernatant
  7. Resuspend the Pellet Mix the Pellet with the remaining Supernatant by repeatedly flipping and tapping it.
  8. Transfer the sample to a 0.2ml PCR tube. You can use a 2-20 or 10-100 Pipette.
  9. Label the tube.