Protocol
https://bento.bio/protocol/biotechnology-101/dna-extraction-from-saliva/
Equipment & Reagents
- Salt
- Eppendorf Tubes
- Paper Cups for collecting Saliva
- Pipette & Tips, 2-20µl or 10-100µl
Protocol
- Label 1.5m Eppendorf Tube with your name
- Prepare Saline (=Salt) solution
Use ca. 5ml of water, mix with a pinch of salt
- Rinse your mouth
- Transfer to 1.5ml Eppendorf Tube.
If you use a Paper Cup, bend it and fill the tube
- Centrifuge at 4KG for 90sec
- Recover the Pellet by decanting the Supernatant
The Pellet is the white concentration of cells on the bottom of the tube. The liquid above is the Supernatant
- Resuspend the Pellet
Mix the Pellet with the remaining Supernatant by repeatedly flipping and tapping it.
- Transfer the sample to a 0.2ml PCR tube.
You can use a 2-20 or 10-100 Pipette.
- Label the tube.